RustEffectorV1, Main, Exploration, bibRecord, 000141

Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene.

Identifieur interne : 000141 ( Main/Exploration ); précédent : 000140; suivant : 000142

Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene.

Auteurs : Gregory J. Lawrence [Australie] ; Peter N. Dodds ; Jeffrey G. Ellis

Source :

The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2010.

RBID : pubmed:19874543

Descripteurs français

KwdFr :
- Basidiomycota (génétique), Basidiomycota (pathogénicité), Extinction de l'expression des gènes (MeSH), Génie génétique (méthodes), Lin (génétique), Lin (microbiologie), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Transformation génétique (MeSH), Virulence (génétique), Végétaux génétiquement modifiés (MeSH).
MESH :
- génétique : Basidiomycota, Lin, Maladies des plantes, Virulence.
- microbiologie : Lin, Maladies des plantes.
- méthodes : Génie génétique.
- pathogénicité : Basidiomycota.
- Extinction de l'expression des gènes, Transformation génétique, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Basidiomycota (genetics), Basidiomycota (pathogenicity), Flax (genetics), Flax (microbiology), Gene Silencing (MeSH), Genetic Engineering (methods), Plant Diseases (genetics), Plant Diseases (microbiology), Plants, Genetically Modified (MeSH), Transformation, Genetic (MeSH), Virulence (genetics).
MESH :
- genetics : Basidiomycota, Flax, Plant Diseases, Virulence.
- methods : Genetic Engineering.
- microbiology : Flax, Plant Diseases.
- pathogenicity : Basidiomycota.
- Gene Silencing, Plants, Genetically Modified, Transformation, Genetic.

Abstract

Rust fungi cause devastating diseases on many important food crops, with a damaging stem rust epidemic currently affecting wheat production in Africa and the Middle East. These parasitic fungi propagate exclusively on plants, precluding the use of many biotechnological tools available for other culturable fungi. In particular the lack of a stable transformation system has been an impediment to the genetic manipulation required for molecular analysis of rust pathogenicity. We have developed an Agrobacterium-mediated genetic transformation procedure for the model flax rust fungus Melampsora lini, which infects flax (Linum usitatissimum). Selection of transgenic rust lines is based on silencing of AvrL567, which encodes a rust effector protein that is recognised by the flax L6 immune receptor. The non-transgenic rust line is unable to infect flax plants expressing L6, while silenced transgenic lines are virulent on these plants, providing an effective selection system. This directly confirms that the cloned AvrL567 gene is responsible for flax rust virulence phenotypes, and demonstrates the utility of this system to probe rust gene function.

DOI: 10.1111/j.1365-313X.2009.04052.x
PubMed: 19874543
PubMed Central: PMC3142615

Affiliations:

Australie

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Lawrence, Gregory J" sort="Lawrence, Gregory J" uniqKey="Lawrence G" first="Gregory J" last="Lawrence">Gregory J. Lawrence</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia. greg.lawrence@csiro.au</nlm:affiliation>
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<author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N" last="Dodds">Peter N. Dodds</name>
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<author><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G" last="Ellis">Jeffrey G. Ellis</name>
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<term>Basidiomycota (pathogenicity)</term>
<term>Flax (genetics)</term>
<term>Flax (microbiology)</term>
<term>Gene Silencing (MeSH)</term>
<term>Genetic Engineering (methods)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
<term>Virulence (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Basidiomycota (pathogénicité)</term>
<term>Extinction de l'expression des gènes (MeSH)</term>
<term>Génie génétique (méthodes)</term>
<term>Lin (génétique)</term>
<term>Lin (microbiologie)</term>
<term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Transformation génétique (MeSH)</term>
<term>Virulence (génétique)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Flax</term>
<term>Plant Diseases</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
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<term>Virulence</term>
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<term>Maladies des plantes</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Flax</term>
<term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génie génétique</term>
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<term>Transformation, Genetic</term>
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<term>Transformation génétique</term>
<term>Végétaux génétiquement modifiés</term>
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<front><div type="abstract" xml:lang="en">Rust fungi cause devastating diseases on many important food crops, with a damaging stem rust epidemic currently affecting wheat production in Africa and the Middle East. These parasitic fungi propagate exclusively on plants, precluding the use of many biotechnological tools available for other culturable fungi. In particular the lack of a stable transformation system has been an impediment to the genetic manipulation required for molecular analysis of rust pathogenicity. We have developed an Agrobacterium-mediated genetic transformation procedure for the model flax rust fungus Melampsora lini, which infects flax (Linum usitatissimum). Selection of transgenic rust lines is based on silencing of AvrL567, which encodes a rust effector protein that is recognised by the flax L6 immune receptor. The non-transgenic rust line is unable to infect flax plants expressing L6, while silenced transgenic lines are virulent on these plants, providing an effective selection system. This directly confirms that the cloned AvrL567 gene is responsible for flax rust virulence phenotypes, and demonstrates the utility of this system to probe rust gene function.</div>
</front>
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	Serveur d'exploration sur les effecteurs de la rouille
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Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene.

Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki